Protein Characterisation Types
Mass
Capillary electrophoresis SDS (CE-SDS)
Quantify protein purity
Determine the molecular weight
Intact Mass Analysis (IMA)
Verify the mass of non-denatured proteins
Detect post-translational modification of proteins
Mass photometry
Measure the molecular mass of proteins and protein complexes in solution
Analyse homogeneity and structural integrity
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify the oligomeric state of proteins
Determine the molar mass of separated peaks
Size
Dynamic Light Scattering (DLS)
Determine the melting temperature of protein
Analyse the thermal stability of a proteins in various formulations
Measure size and size distribution
Estimate polydispersity
Quantify the type and degree of aggregations
Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)
Fractionate macromolecules and measure molar mass and size distributions
Estimate polydispersity
Analyse structural integrity
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify the oligomeric state of proteins
Determine the molar mass of separated peaks
Transmission Electron Microscopy (TEM)
Visualise negatively stained macromolecular structures
Homogeneity
Dynamic Light Scattering (DLS)
Determine the melting temperature of proteins
Analyse the thermal stability of a protein in various formulations
Measure size and size distribution
Estimate polydispersity
Quantify the type and degree of aggregations
Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)
Fractionate macromolecules and measure molar mass and size distributions
Estimate polydispersity
Analyse structural integrity
Mass photometry
Measure the molecular mass of proteins and protein assemblies in solution
Analyse homogeneity and structural integrity
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify the oligomeric state of proteins
Determine the molar mass of separate peaks
Stability
Dynamic Light Scattering (DLS)
Determine the melting temperature of proteins
Analyse the thermal stability of a protein in various formulations
Measure size and size distribution
Estimate polydispersity
Quantify the type and degree of aggregations
Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)
Fractionate macromolecules and measure molar mass and size distributions
Estimate polydispersity
Analyse structural integrity
Mass photometry
Measure the molecular mass of proteins and protein assemblies in solution
Analyse homogeneity and structural integrity
Nano Differential Scanning Fluorometry (nanoDSF)
Analyse the thermal stability of a protein in various formulations
Determine the precise unfolding temperature
Determine aggregation onset temperature
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify the oligomeric state of proteins
Determine the molar mass of separated peaks
Transmission Electron Microscopy (TEM)
Visualise negatively stained macromolecular structures
Circular Dichroism (CD) Spectroscopy
Determine the secondary structure content of proteins
Assess inclusion body refolding strategies
Analyse the structural stability of proteins in various formulations