E. coli is one of the most widely used expression hosts for the production of recombinant proteins.

The advantages of this system include:

  • Inexpensive setup and running costs
  • High recombinant protein production levels 
  • Short timeframe for protein expression
  • Limited technical knowledge required for culturing
  • Scalability from small (1 mL) to very large culture (>10,000 L) volumes

One of the main drawbacks of this expression system however is its limited capability for post-translational modifications (PTMs).