Protein characterisation

Protein quality is central to data integrity and reproducibility.

PEF has the expertise in analysing a broad range of biomolecules from recombinant or native source, including peptides, antibodies and virus-like particles. Proteins produced in PEF are characterised to verify the molar mass, size, homogeneity, identity, purity and stability. Additional attributes are investigated for different classes of proteins to provide a comprehensive profile of the protein of interest. Orthogonal methods are applied to characterise a given attribute to provide the protein properties.

Protein Attributes

Charge Protein Characteristion - PEF
Charge
Homogeniety  Protein Characteristion - PEF
Homogeneity
Identity Protein Characteristion - PEF
Identity
Molar Mass  Protein Characteristion - PEF
Mass
Protein size Protein Characteristion - PEFSize
Structural integrity Protein Characteristion - PEF
Structural integrity

Protein Analysis Capabilities

Protein Identity

Peptide Mass Fingerprinting (PMF)

Verify protein sequence, compare known and acquired sequence


SDS-PAGE analysis

Qualitative analysis on protein purity and molecular weight


Western blot analysis

Verify band of interest

 

Mass

Capillary electrophoresis SDS (CE-SDS)

Quantify protein purity

Determine molecular weight


Intact Mass Analysis (IMA)

Verify the mass of the non-denatured protein

Detect post-translational modification on protein


Mass photometry​

Measure the molecular mass of proteins and protein assemblies in solution

Analyse homogeneity and structural integrity


Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks

Size

Dynamic Light Scattering (DLS)​

Determine melting temperature of protein

Analyse thermal stability of a protein in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify type and degree of aggregations


Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity


Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks


Transmission Electron Microscopy (TEM)

Visualise negatively stained macromolecular structures

Charge

Capillary isoelectric focussing (cIEF)

Separate biomolecules according to isoelectric point

Analyse charge heterogeneity


Cation-exchange high performance liquid chromatography (CEX-HPLC)

Separate and quantify biomolecules based on charge

Determine the presence of charge variants

Homogeneity

Dynamic Light Scattering (DLS)

Determine melting temperature of protein

Analyse thermal stability of a protein in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify type and degree of aggregations


Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity


Mass photometry

Measure the molecular mass of proteins and protein assemblies in solution

Analyse homogeneity and structural integrity


Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks

Stability

Dynamic Light Scattering (DLS)

Determine melting temperature of protein

Analyse thermal stability of a protein in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify type and degree of aggregations


Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity


Mass photometry

Measure the molecular mass of proteins and protein assemblies in solution

Analyse homogeneity and structural integrity


Nano Differential Scanning Fluorometry (nanoDSF)

Analyse thermal stability of a protein in various formulations

Determine precise unfolding temperature

Determine aggregation onset temperature


Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks


Transmission Electron Microscopy (TEM)

Visualise negatively stained macromolecular structures

Quantity

A280

Measure total protein concentration


Bicinchoninic acid (BCA) calorimetric assay

Measure total protein concentration


Protein A high performance liquid chromatography (Protein A HPLC)

Measure antibody titre

For all characterisation services, please contact us.
To view our list of reagents, please visit our UQ Resource Centre.

Training

PEF offers upskilling for staff and students in all aspects of recombinant protein production.
If you would like to enquire about training in any of the services offered by PEF please contact us.

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