Protein characterisation

PEF offers a number of characterisation services for analysing recombinant proteins or virus-like particles (VLPs). Services available from PEF include:

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)

Separates proteins based on electrophoretic mobility. Estimate protein molecular weight and purity

Analytical size exclusion chromatography (SEC-HPLC)

Estimates native protein molecular weight, determine multimeric structure and degree of aggregation

Dynamic light scattering (DLS)

Determines size distribution profile of proteins and protein complexes. Suitable for high throughput detection of protein aggregation, thermal stability and storage conditions

Western immunoblot

Identifies protein using immunological specificity for amino acid sequence or epitope

Endotoxin measurement

Measures endotoxin level (required for all samples destined for animal studies)

Asymmetric flow-field flow-fractionation (AF4)

Accurately determines size distribution profile of VLPs and protein complexes

Bicinchoninic acid (BCA) assay

Determines total protein concentration

Intact protein analysis

Accurately determines native protein molecular weight

Peptide mass fingerprint (PMF)

Identifies purified protein or excised band from polyacrylamide gel

Transmission electron microscopy (TEM)

Enables imaging and morphological characterisation of VLPs and protein complexes

For all characterisation services, please contact us.
To view our list of available vectors, please visit our UQ Resource Centre.

Training

PEF offers upskilling for staff and students in all aspects of recombinant protein production.
If you would like to enquire about training in any of the services offered by PEF please contact us.

Contact Us