Protein Characterisation Types

Capillary electrophoresis SDS (CE-SDS)

Quantify protein purity

Determine molecular weight

Intact Mass Analysis (IMA)

Verify the mass of the non-denatured protein

Detect post-translational modification on protein

Mass photometry​

Measure the molecular mass of proteins and protein assemblies in solution

Analyse homogeneity and structural integrity

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks

Dynamic Light Scattering (DLS)​

Determine melting temperature of protein

Analyse thermal stability of a protein in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify type and degree of aggregations

Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks

Transmission Electron Microscopy (TEM)

Visualise negatively stained macromolecular structures

Capillary isoelectric focussing (cIEF)

Separate biomolecules according to isoelectric point

Analyse charge heterogeneity

Cation-exchange high performance liquid chromatography (CEX-HPLC)

Separate and quantify biomolecules based on charge

Determine the presence of charge variants

Dynamic Light Scattering (DLS)

Determine melting temperature of protein

Analyse thermal stability of a protein in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify type and degree of aggregations

Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity

Mass photometry

Measure the molecular mass of proteins and protein assemblies in solution

Analyse homogeneity and structural integrity

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks

Dynamic Light Scattering (DLS)

Determine melting temperature of protein

Analyse thermal stability of a protein in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify type and degree of aggregations

Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity

Mass photometry

Measure the molecular mass of proteins and protein assemblies in solution

Analyse homogeneity and structural integrity

Nano Differential Scanning Fluorometry (nanoDSF)

Analyse thermal stability of a protein in various formulations

Determine precise unfolding temperature

Determine aggregation onset temperature

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify oligomeric state of protein

Determine molar mass of separate peaks

Transmission Electron Microscopy (TEM)

Visualise negatively stained macromolecular structures

A280

Measure total protein concentration

Bicinchoninic acid (BCA) calorimetric assay

Measure total protein concentration

Protein A high performance liquid chromatography (Protein A HPLC)

Measure antibody titre