Protein Characterisation Types
Mass
Capillary electrophoresis SDS (CE-SDS)
Quantify protein purity
Determine molecular weight
Intact Mass Analysis (IMA)
Verify the mass of the non-denatured protein
Detect post-translational modification on protein
Mass photometry
Measure the molecular mass of proteins and protein assemblies in solution
Analyse homogeneity and structural integrity
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify oligomeric state of protein
Determine molar mass of separate peaks
Size
Dynamic Light Scattering (DLS)
Determine melting temperature of protein
Analyse thermal stability of a protein in various formulations
Measure size and size distribution
Estimate polydispersity
Quantify type and degree of aggregations
Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)
Fractionate macromolecules and measure molar mass and size distributions
Estimate polydispersity
Analyse structural integrity
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify oligomeric state of protein
Determine molar mass of separate peaks
Transmission Electron Microscopy (TEM)
Visualise negatively stained macromolecular structures
Homogeneity
Dynamic Light Scattering (DLS)
Determine melting temperature of protein
Analyse thermal stability of a protein in various formulations
Measure size and size distribution
Estimate polydispersity
Quantify type and degree of aggregations
Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)
Fractionate macromolecules and measure molar mass and size distributions
Estimate polydispersity
Analyse structural integrity
Mass photometry
Measure the molecular mass of proteins and protein assemblies in solution
Analyse homogeneity and structural integrity
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify oligomeric state of protein
Determine molar mass of separate peaks
Stability
Dynamic Light Scattering (DLS)
Determine melting temperature of protein
Analyse thermal stability of a protein in various formulations
Measure size and size distribution
Estimate polydispersity
Quantify type and degree of aggregations
Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)
Fractionate macromolecules and measure molar mass and size distributions
Estimate polydispersity
Analyse structural integrity
Mass photometry
Measure the molecular mass of proteins and protein assemblies in solution
Analyse homogeneity and structural integrity
Nano Differential Scanning Fluorometry (nanoDSF)
Analyse thermal stability of a protein in various formulations
Determine precise unfolding temperature
Determine aggregation onset temperature
Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)
Verify oligomeric state of protein
Determine molar mass of separate peaks
Transmission Electron Microscopy (TEM)
Visualise negatively stained macromolecular structures