Protein Purification

Design an effective purification strategy to obtain high purity functional protein.

PEF utilises a wide range of capabilities to purify different types of protein from both recombinant and native sources. Taking into consideration the end-use of the protein, a purification strategy is designed to achieve high purity and stable protein. PEF conducts small-scale screening to determine optimal resin and buffer, develops multi-step purification process and operates endotoxin-free purification.

Chromatography techniques


Hydrophobic interaction


Reverse phase

Size exclusion


Non-chromatography techniques

Dead-end filtration

Density gradient ultracentrifugation

Protein precipitation

Tangential flow filtration

Sterile filtration

Proteases for fusion tag removal

Enterokinase protease

Human Rhinovirus (HRV) 3C protease

Thrombin protease

Tobacco Etch Virus (TEV) protease

Small Ubiquitin-Like Modifier (SUMO) protease

Impurity clearance

Aggregates removal

Endotoxin removal

Host cell DNA removal

Host cell protein removal

Process scale-up

Column packing

Column integrity testing

Protein formulation

Protein concentration

Buffer exchange

For protein purification services, please contact us for a quote.

To view our list of reagents, please visit our UQ Resource Centre.


PEF offers upskilling for staff and students in all aspects of recombinant protein production.
If you would like to enquire about training in any of the services offered by PEF please contact us.

Contact Us