Protein Purification
Design an effective purification strategy to obtain high purity functional protein.
PEF utilises a wide range of capabilities to purify different types of protein from both recombinant and native sources. Taking into consideration the end-use of the protein, a purification strategy is designed to achieve high purity and stable protein. PEF conducts small-scale screening to determine optimal resin and buffer, develops multi-step purification process and operates endotoxin-free purification.
Chromatography techniques
Affinity
Hydrophobic interaction
Ion-exchange
Reverse phase
Size exclusion
Multi-modal
Non-chromatography techniques
Dead-end filtration
Density gradient ultracentrifugation
Protein precipitation
Tangential flow filtration
Sterile filtration
Proteases for fusion tag removal
Enterokinase protease
Human Rhinovirus (HRV) 3C protease
Thrombin protease
Tobacco Etch Virus (TEV) protease
Small Ubiquitin-Like Modifier (SUMO) protease
Impurity clearance
Aggregates removal
Endotoxin removal
Host cell DNA removal
Host cell protein removal
Process scale-up
Column packing
Column integrity testing
Protein formulation
Protein concentration
Buffer exchange
For protein purification services, please contact us for a quote.
To view our list of reagents, please visit our UQ Resource Centre.
Training
PEF offers upskilling for staff and students in all aspects of recombinant protein production.
If you would like to enquire about training in any of the services offered by PEF please contact us.