UQ Resource Centre
The UQ Resource Centre within the Protein Expression Facility (PEF) provides quality‑assured materials and reagents to support research involving molecular cloning, protein expression, purification and protein modification for the UQ research community.
For pricing information, please email pef@uq.edu.au.
To submit an order, please complete a Request for Internal Services Quote.
Plasmids
PEF can provide a streaked plate containing the plasmid of interest or a plasmid miniprep.
Parallel expression vectors (E. coli, Baculovirus/insect cell and Mammalian cell)
- Non-fluorescent vectors
- Fluorescent vectors
Bacterial expression vectors
- pET vector series
- pGEX vector series
- pDEST vector series
Baculovirus/insect cell transfer vectors
Mammalian expression vectors
Strains and Cells
Bacterial strain
PEF can provide a bacterial-streaked plate or an aliquot of chemically competent cells.
- XL1 Blue
- Top10
- JM109
- DH5 alpha
- Omnimax
- Shuffle T7 Express
- DH10Bac
- BL21 DE3
- BL21 DE3 Star
- BL21 DE3 RIL
- BL21 DE3 pLysS
- Rosetta DE3 pLysS
- Rosetta 2 DE3
- Tuner DE3
- Arctic Express DE3 RIL
Insect cells
- Sf9
- High FiveTM
Mammalian cells
- CHO cell lines
- HEK293 cell lines
Media
Insect cell
- ESF 921
Enzymes
PEF produces enzymes in-house to support molecular cloning, protein expression, and purification applications. Please contact us for more details.
- TEV Protease
- HRV3C Protease
- SUMO Protease
- Recombinant Benzonase (tag-free)
- Taq Polymerase (>2.5 U/µL)
- BirA Biotin Protein Ligase (cleavable GST tag)