Expression Optimisation

Select the right expression platform for the functionality of the recombinant protein.

PEF has extensive experience and capabilities in using Escherichia coli, yeast, baculovirus/insect cell, mammalian cell and cell-free in vitro platform technologies to produce a wide range of proteins. Parallel screening of expression parameters at the start of the project delivers high-value data in a short timeframe that will enhance the success of the protein production.

Parallel expression optimisation

Cost-effective and rapid high-throughput screen to achieve optimal protein expression and protein solubility

Multiple expression platforms

Optimal expression platform for heterologous production of protein of interest with desired functionality

Expression technologies

Multiple technologies within each expression platform to maximise protein production

 
Low
High

Cost

Bacteria
Bacteria
 
Yeast
Yeast
Baculovirus / Insect cell
Baculovirus / Insect cell
Mammalian
Mammalian
 

Speed

Mammalian
Mammalian
 
Baculovirus / Insect cell
Baculovirus / Insect cell
Yeast
Yeast
Bacteria
Bacteria
 

Yield

Mammalian
Mammalian
 
Baculovirus / Insect cell
Baculovirus / Insect cell
Bacteria
Bacteria
Yeast
Yeast
 

Protein Complex

Bacteria
Bacteria
 
Yeast
Yeast
Mammalian
Mammalian
Baculovirus / Insect cell
Baculovirus / Insect cell

For all expression optimisation services, please contact us.
To view our list of reagents, please visit our UQ Resource Centre.

Training

PEF offers upskilling for staff and students in all aspects of recombinant protein production.
Training to independently access our Ambr250 bioreactor process development equipment is available.
If you would like to enquire about training in any of the services offered by PEF, please contact us.

Contact Us