Research services

PEF production platform

The PEF production platform provides a quick snapshot of the services we offer to help fast-track your research.  From the Gene-to-Protein complete package to any stage in-between; we can customise to suit your project. Contact us to discuss how we can support your research.


Molecular Engineering

  • Capabilities in numerous cloning strategies and access to a suite of vectors
  • Customised or high-throughput services for gene and vector engineering
  • Endotoxin-free plasmid production

Expression Optimisation

  • Rapid high-throughput screen to achieve optimal protein expression, solubility or secretion
  • Expression in four host systems: bacteria, yeast, baculovirus-insect cell and mammalian
  • Multiple technologies within each host system to maximise protein production

Scale-up Expression

  • Scale-up expression of protein in shake flask (1 - 20 L) in all four host systems
  • Bacteria and yeast fermentation in stirred tank bioreactor (1 - 20 L)
  • Insect and mammalian cell culture in rocking motion bioreactor (1 - 25 L)

Purification

  • Capabilities in isolation of intracellular, membrane and secretory proteins
  • Chromatographic and non-chromatographic separation technologies
  • Endotoxin-free protein preparation

Characterisation

  • Protein concentration, purity, intact mass, identification and sequencing
  • Protein stability and formulation to maximise functionality
  • Protein conformation and structural integrity analysis