PEF production platform
The PEF production platform provides a quick snapshot of the services we offer to help fast-track your research. From the Gene-to-Protein complete package to any stage in-between; we can customise to suit your project. Contact us to discuss how we can support your research.
Molecular Engineering
- Capabilities in numerous cloning strategies and access to a suite of vectors
- Customised or high-throughput services for gene and vector engineering
- Endotoxin-free plasmid production
Expression Optimisation
- Rapid high-throughput screen to achieve optimal protein expression, solubility or secretion
- Expression in four host systems: bacteria, yeast, baculovirus-insect cell and mammalian
- Multiple technologies within each host system to maximise protein production
Scale-up Expression
- Scale-up expression of protein in shake flask (1 - 20 L) in all four host systems
- Bacteria and yeast fermentation in stirred tank bioreactor (1 - 20 L)
- Insect and mammalian cell culture in rocking motion bioreactor (1 - 25 L)
Purification
- Capabilities in isolation of intracellular, membrane and secretory proteins
- Chromatographic and non-chromatographic separation technologies
- Endotoxin-free protein preparation
Analytics and Characterisation
- Protein concentration, purity, intact mass, identification and sequencing
- Protein stability and formulation to maximise functionality
- Protein conformation and structural integrity analysis