Protein Characterisation Types

Capillary electrophoresis SDS (CE-SDS)

(Training for independent access available)

Quantify protein purity

Determine the molecular weight

Intact Mass Analysis (IMA)

Verify the mass of non-denatured proteins

Detect post-translational modification of proteins

Mass photometry​

Measure the molecular mass of proteins and protein complexes in solution

Analyse homogeneity and structural integrity

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify the oligomeric state of proteins

Determine the molar mass of separated peaks

Dynamic Light Scattering (DLS)​

(Training for independent access available)

Determine the melting temperature of protein

Analyse the thermal stability of a proteins in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify the type and degree of aggregations

Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

(Training for independent access available)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify the oligomeric state of proteins

Determine the molar mass of separated peaks

Transmission Electron Microscopy (TEM)

Visualise negatively stained macromolecular structures

Capillary isoelectric focusing (cIEF)

(Training for independent access available)

Separate biomolecules according to their isoelectric point

Analyse charge heterogeneity

Cation-exchange high-performance liquid chromatography (CEX-HPLC)

Separate and quantify biomolecules based on charge

Determine the presence of charge variants

Dynamic Light Scattering (DLS)

(Training for independent access available)

Determine the melting temperature of proteins

Analyse the thermal stability of a protein in various formulations

Measure size and size distribution

Estimate polydispersity

Quantify the type and degree of aggregations

Field-flow fractionation coupled with multi-angle light scattering (FFF-MALS)

(Training for independent access available)

Fractionate macromolecules and measure molar mass and size distributions

Estimate polydispersity

Analyse structural integrity

Mass photometry

Measure the molecular mass of proteins and protein assemblies in solution

Analyse homogeneity and structural integrity

Size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS)

Verify the oligomeric state of proteins

Determine the molar mass of separate peaks