Professor David J. Owen
Director, Protein Expression Facility
The University of Queensland
+61 7 3346 3498
+61 422 700 331
David is a passionate and driven scientist with a successful track record in translational commercially focused technical and academic leadership. David has worked in academia and industry at the interface of chemistry, biochemistry and biology. He has successfully designed and developed several oncology nanomedicines and driven them forward into clinical trials.
As Director of the Protein Expression Facility (PEF) within The University of Queensland, David and his team strive to build collaborations and provide excellent service provision with a wide variety of researchers across academia and industry. Through this work we ensure PEF achieves its vision to be a world leader in protein research services and innovative solutions for protein production driving scientific success.
Journal Articles
Sonzini, Silvia, Caputo, Fanny, Mehn, Dora, Calzolai, Luigi, Even Borgos, Sven, Hyldbakk, Astrid, Treacher, Kevin, Li, Weimin, Jackman, Mark, Mahmoudi, Najet, Jayne Lawrence, M., Patterson, Claire, Owen, David, Ashford, Marianne and Akhtar, Nadim (2023). In depth characterization of physicochemical critical quality attributes of a clinical drug-dendrimer conjugate. International Journal of Pharmaceutics, 637 122905, 1-14. doi: 10.1016/j.ijpharm.2023.122905
Feeney, Orlagh M., Ardipradja, Katie, Noi, Ka Fung, Mehta, Dharmini, De Rose, Robert, Yuen, Daniel, Johnston, Angus P.R., Kingston, Lee, Ericsson, Cecilia, Elmore, Charles S., Hufton, Richard, Owen, David J., Ashford, Marianne B. and Porter, Christopher J.H. (2022). Subcutaneous delivery of a dendrimer-BH3 mimetic improves lymphatic uptake and survival in lymphoma. Journal of Controlled Release, 348, 420-430. doi: 10.1016/j.jconrel.2022.05.041
Patterson, Claire M., Balachander, Srividya B., Grant, Iain, Pop-Damkov, Petar, Kelly, Brian, McCoull, William, Parker, Jeremy, Giannis, Michael, Hill, Kathryn J., Gibbons, Francis D., Hennessy, Edward J., Kemmitt, Paul, Harmer, Alexander R., Gales, Sonya, Purbrick, Stuart, Redmond, Sean, Skinner, Matthew, Graham, Lorraine, Secrist, J. Paul, Schuller, Alwin G., Wen, Shenghua, Adam, Ammar, Reimer, Corinne, Cidado, Justin, Wild, Martin, Gangl, Eric, Fawell, Stephen E., Saeh, Jamal, Davies, Barry R. ... Ashford, Marianne B. (2021). Design and optimisation of dendrimer-conjugated Bcl-2/xL inhibitor, AZD0466, with improved therapeutic index for cancer therapy. Communications Biology, 4 (1) 112, 112. doi: 10.1038/s42003-020-01631-8
Mehta, Dharmini, Leong, Nathania, McLeod, Victoria M., Kelly, Brian D., Pathak, Rashmi, Owen, David J., Porter, Christopher J.H. and Kaminskas, Lisa M. (2018). Reducing dendrimer generation and PEG chain length increases drug release and promotes anti-cancer activity of PEGylated polylysine dendrimers conjugated with doxorubicin via a cathepsin-cleavable peptide linker. Molecular Pharmaceutics, 15 (10) acs.molpharmaceut.8b00581, 4568-4576. doi: 10.1021/acs.molpharmaceut.8b00581
Leong, Nathania J., Mehta, Dharmini, McLeod, Victoria M., Kelly, Brian D., Pathak, Rashmi, Owen, David J., Porter, Christopher J. H. and Kaminskas, Lisa M. (2018). Doxorubicin conjugation and drug linker chemistry alter the intravenous and pulmonary pharmacokinetics of a PEGylated Generation 4 polylysine dendrimer in rats. Journal of Pharmaceutical Sciences, 107 (9), 2509-2513. doi: 10.1016/j.xphs.2018.05.013
Ryan G.M., McLeod V.M., Mehta D., Kelly B.D., Stanislawski P.C., Owen D.J., Kaminskas L.M. and Porter C.J.H. (2017). Lymphatic transport and lymph node targeting of methotrexate-conjugated PEGylated dendrimers are enhanced by reducing the length of the drug linker or masking interactions with the injection site. Nanomedicine: Nanotechnology, Biology and Medicine, 13 (8), 2485-2494. doi: 10.1016/j.nano.2017.08.003
Haque, Shadabul, McLeod, Victoria M., Jones, Seth, Fung, Sandy, Whittaker, Michael, McIntosh, Michelle, Pouton, Colin, Owen, David J., Porter, Christopher J. H. and Kaminskas, Lisa M. (2017). Effect of increased surface hydrophobicity via drug conjugation on the clearance of inhaled PEGylated polylysine dendrimers. European Journal of Pharmaceutics and Biopharmaceutics, 119, 408-418. doi: 10.1016/j.ejpb.2017.07.005
Ryan, Gemma M., Bischof, Robert J., Enkhbaatar, Perenlei, McLeod, Victoria M., Chan, Linda J., Jones, Seth A., Owen, David J., Porter, Christopher J. H. and Kaminskas, Lisa M. (2016). A comparison of the pharmacokinetics and pulmonary lymphatic exposure of a generation 4 PEGylated dendrimer following intravenous and aerosol administration to rats and sheep. Pharmaceutical Research, 33 (2), 510-525. doi: 10.1007/s11095-015-1806-z
Kaminskas, Lisa M., McLeod, Victoria M., Ascher, David B., Ryan, Gemma M., Jones, Seth, Haynes, John M., Trevaskis, Natalie L., Chan, Linda J., Sloan, Erica K., Finnin, Benjamin A., Williamson, Mark, Velkov, Tony, Williams, Elizabeth D., Kelly, Brian D., Owen, David J. and Porter, Christopher J. H. (2015). Methotrexate-conjugated PEGylated dendrimers show differential patterns of deposition and activity in tumor-burdened lymph nodes after intravenous and subcutaneous administration in rats. Molecular Pharmaceutics, 12 (2), 432-443. doi: 10.1021/mp500531e
Kaminskas, Lisa M., McLeod, Victoria M., Ryan, Gemma M., Kelly, Brian D., Haynes, John M., Williamson, Mark, Thienthong, Neeranat, Owen, David J. and Porter, Christopher J. H. (2014). Pulmonary administration of a doxorubicin-conjugated dendrimer enhances drug exposure to lung metastases and improves cancer therapy. Journal of Controlled Release, 183 (1), 18-26. doi: 10.1016/j.jconrel.2014.03.012
Ryan, Gemma M., Kaminskas, Lisa M., Bulitta, Juergen B., McIntosh, Michelle P., Owen, David J. and Porter, Christopher J. H. (2013). PEGylated polylysine dendrimers increase lymphatic exposure to doxorubicin when compared to PEGylated liposomal and solution formulations of doxorubicin. Journal of Controlled Release, 172 (1), 128-136. doi: 10.1016/j.jconrel.2013.08.004
Ryan, Gemma M., Kaminskas, Lisa M., Kelly, Brian D., Owen, David J., McIntosh, Michelle P. and Porter, Christopher J. H. (2013). Pulmonary administration of PEGylated polylysine dendrimers: absorption from the lung versus retention within the lung is highly size-dependent. Molecular Pharmaceutics, 10 (8), 2986-2995. doi: 10.1021/mp400091n
Kaminskas, Lisa M., McLeod, Victoria M., Kelly, Brian D., Cullinane, Carleen, Sberna, Gian, Williamson, Mark, Boyd, Ben J., Owen, David J. and Porter, Christopher J. H. (2012). Doxorubicin-conjugated PEGylated dendrimers show similar tumoricidal activity but lower systemic toxicity when compared to PEGylated liposome and solution formulations in mouse and rat tumor models. Molecular Pharmaceutics, 9 (3), 422-432. doi: 10.1021/mp200522d
Kaminskas, Lisa M., McLeod, Victoria M., Kelly, Brian D., Sberna, Gian, Boyd, Ben J., Williamson, Mark, Owen, David J. and Porter, Christopher J. H. (2012). A comparison of changes to doxorubicin pharmacokinetics, antitumor activity, and toxicity mediated by PEGylated dendrimer and PEGylated liposome drug delivery systems. Nanomedicine: Nanotechnology, Biology and Medicine, 8 (1), 103-111. doi: 10.1016/j.nano.2011.05.013
Kaminskas, Lisa M., Kelly, Brian D., McLeod, Victoria M., Sberna, Gian, Owen, David J., Boyd, Ben J. and Porter, Christopher J. H. (2011). Characterisation and tumour targeting of PEGylated polylysine dendrimers bearing doxorubicin via a pH labile linker. Journal of Controlled Release, 152 (2), 241-248. doi: 10.1016/j.jconrel.2011.02.005
Kaminskas, Lisa M., Kelly, Brian D., McLeod, Victoria M., Sberna, Gian, Boyd, Ben J., Owen, David J. and Porter, Christopher J. H. (2011). Capping methotrexate α-carboxyl groups enhances systemic exposure and retains the cytotoxicity of drug conjugated pegylated polylysine dendrimers. Molecular Pharmaceutics, 8 (2), 338-349. doi: 10.1021/mp1001872
Owen, David and Barrett, Paul (2010). Dendrimers enhance delivery of small molecule and biological drugs: Control solubility, half-life, toxicity and targeting. ONdrugDelivery (4), 21-24.
Koohang, Ali, Bailey, Jessica L., Coates, Robert M., Erickson, Hans K., Owen, David and Poulter, C. Dale (2010). Enantioselective inhibition of squalene synthase by aziridine analogues of presqualene diphosphate. Journal of Organic Chemistry, 75 (14), 4769-4777. doi: 10.1021/jo100718z
Adams, Tim E., Sous, Mariana El, Hawkins, Bill C., Hirner, Sebastian, Holloway, Georgina, Khoo, Mui Ling, Owen, David J., Paul Savage, G., Seammells, Peter J. and Rizzacasa, Mark A. (2009). Total Synthesis of the Potent Anticancer Aglaia Metabolites (-)-Silvestrol and (-)-Episilvestrol and the Active Analogue (-)-4-Desmethoxyepisilvestrol. Journal of the American Chemical Society, 131 (4), 1607-1616. doi: 10.1021/ja808402e
El Sous, Mariana, Khoo, Mui Ling, Holloway, Georgina, Owen, David, Scammells, Peter J. and Rizzacasa, Mark A. (2007). Total synthesis of (-)-episilvestrol and (-)-silvestrol. Angewandte Chemie - International Edition, 46 (41), 7835-7838. doi: 10.1002/anie.200702700
Davis, Chris B., Hartnell, Regan D., Madge, Paul D., Owen, David J., Thomson, Robin J., Chong, Andrew K.J., Coppel, Ross L. and Von Itzstein, Mark (2007). Synthesis and biological evaluation of galactofuranosyl alkyl thioglycosides as inhibitors of mycobacteria. Carbohydrate Research, 342 (12-13), 1773-1780. doi: 10.1016/j.carres.2007.04.027
Owen, David J., Davis, Chris B., Hartnell, Regan D., Madge, Paul D., Thomson, Robin J., Chong, Andrew K.J., Coppel, Ross L. and Von Itzstein, Mark (2007). Synthesis and evaluation of galactofuranosyl N,N-dialkyl sulfenamides and sulfonamides as antimycobacterial agents. Bioorganic and Medicinal Chemistry Letters, 17 (8), 2274-2277. doi: 10.1016/j.bmcl.2007.01.068
Pei, Yazhong, Lilly, Michael J., Owen, David J., D'Souza, Lawrence J., Tang, Xiao-Qing, Yu, Jinghua, Nazarbaghi, Ramina, Hunter, Andrew, Anderson, Christen M., Glasco, Susan, Ede, Nicholas J., James, Ian W., Maitra, Uday, Chandrasekaran, S., Moos, Walter H. and Ghosh, Soumitra S. (2003). Efficient syntheses of benzothiazepines as antagonists for the mitochondrial sodium-calcium exchanger: Potential therapeutics for type II diabetes. Journal of Organic Chemistry, 68 (1), 92-103. doi: 10.1021/jo020446t
Owen, David J., Thomson, Robin J. and Von Itzstein, Mark (2002). A convenient synthesis of C-galactofuranosylic compounds (C-galactofuranosides). Carbohydrate Research, 337 (21-23), 2017-2022. doi: 10.1016/S0008-6215(02)00133-7
Kuhn, Karsten, Owen, David J., Bader, Benjamin, Wittinghofer, Alfred, Kuhlmann, Jürgen and Waldmann, Herbert (2001). Synthesis of Functional Ras Lipoproteins and Fluorescent Derivatives. Journal of the American Chemical Society, 123 (6), 1023-1035. doi: 10.1021/ja002723o
Blake, Patrick S, Taylor, David R, Crisp, Carol M, Mander, Lewis N and Owen, David J (2000). Identification of endogenous gibberellins in strawberry, including the novel gibberellins GA123, GA124 and GA125. Phytochemistry, 55 (8), 887-890. doi: 10.1016/S0031-9422(00)00237-5
Thoma N.H., Iakovenko A., Owen D., Scheidig A.S., Waldmann H., Goody R.S. and Alexandrov K. (2000). Phosphoisoprenoid binding specificity of geranylgeranyltransferase type II. Biochemistry, 39 (39), 12043-12052. doi: 10.1021/bi000835m
Owen, David J. and Von Itzstein, Mark (2000). A one-pot synthesis of novel N,N-dialkyl-S-glycosylsulfenamides. Carbohydrate Research, 328 (3), 287-292. doi: 10.1016/S0008-6215(00)00110-5
Bader, Benjamin, Kuhn, Karsten, Owen, David J., Waldmann, Herbert, Wittinghofer, Alfred and Kuhlmann, Jürgen (2000). Bioorganic synthesis of lipid-modified proteins for the study of signal transduction. Nature, 403 (6766), 223-226. doi: 10.1038/35003249
Eisele, Frank, Owen, David J. and Waldmann, Herbert (1999). Peptide conjugates as tools for the study of biological signal transduction. Bioorganic and Medicinal Chemistry, 7 (2), 193-224. doi: 10.1016/S0968-0896(98)00204-1
Koohang, Ali, Coates, Robert M., Owen, David and Poulter, C. Dale (1999). Synthesis and evaluation of aziridine analogues of presqualene diphosphate as squalene synthase inhibitors. Journal of Organic Chemistry, 64 (1), 6-7. doi: 10.1021/jo981833z
Owen, DJ, Alexandrov, K, Rostkova, E, Scheidig, AJ, Goody, RS and Waldmann, H (1999). Chemo-enzymatic synthesis of fluorescent Rab 7 proteins: Tools to study vesicular trafficking in cells. Angewandte Chemie-International Edition, 38 (4), 509-512. doi: 10.1002/(SICI)1521-3773(19990215)38:4<509::AID-ANIE509>3.3.CO;2-V
Nakayama, Massyoshi, Nishijima, Takaaki, Koshioka, Masaji, Yamane, Hisakazu, Owen, David J. and Mander, Lewis N. (1998). Identification of GA113, GA114, GA115 and GA116 and further novel gibberellins in Raphanus sativus. Phytochemistry, 48 (4), 587-593. doi: 10.1016/s0031-9422(98)00035-1
Owen, David J., Mander, Lewis N., Storey, John M.D., Huntley, Rachael P., Gaskin, Paul, Lenton, John R., Gage, Douglas A. and Zeevaart, Jan A.D. (1998). Synthesis and confirmation of structure for a new gibberellin, 2β- hydroxy-GA12 (GA110), from spinach and oil palm. Phytochemistry, 47 (3), 331-337. doi: 10.1016/S0031-9422(97)00577-3
Mander, Lewis N. and Owen, David J. (1997). Synthesis of 12-hydroxy C20-gibberellins. Tetrahedron, 53 (6), 2137-2162. doi: 10.1016/s0040-4020(96)01109-x
Mander, Lewis N., Owen, David J., Croker, Stephen J., Gaskin, Paul, Hedden, Peter, Lewis, Mervyn J., Talon, Manuel, Gage, Douglas A., Zeevaart, Jan A.D., Brenner, Mark L. and Sheng, Chuxing (1996). Identification of three C20-gibberellins: GA97 (2β-hydroxy-GA53), GA98 (2β-hydroxy-GA44) and GA99 (2β-hydroxy-GA19). Phytochemistry, 43 (1), 23-28. doi: 10.1016/0031-9422(96)00251-8
Owen, David J., Mander, Lewis N., Gaskin, Paul and Macmillan, Jake (1996). Synthesis and confirmation of structure of three 13,15β-dihydroxy C-20 gibberellins, GA100, GA101 and GA102, isolated from the seeds of Helianthus annuus L. Phytochemistry, 42 (4), 921-925. doi: 10.1016/0031-9422(96)00045-3
Mander, Lewis N. and Owen, David J. (1996). Structure determination and synthesis of a new gibberellin, GA99, from spinach plants: 2β-hydroxy-GA19. Tetrahedron Letters, 37 (5), 723-726. doi: 10.1016/0040-4039(95)02252-X
Mander, Lewis N., Owen, David J. and Twitchin, Bruce (1996). Synthesis of gibberellin GA53 and its (17,17-D2)derivative. Australian Journal of Chemistry, 49 (2), 249-253. doi: 10.1071/ch9960249
Yamauchi, Tadayuki, Oyama, Naomi, Yamane, Hisakazu, Murofushi, Noboru, Schraudolf, Helmut, Owen, David and Mander, Lewis N. (1995). 3-epi-GA63, antheridiogen in Anemia phyllitidis. Phytochemistry, 38 (6), 1345-1348. doi: 10.1016/0031-9422(94)00834-G
Wentrup C., Kambouris P., Evans R.A., Owen D., Macfarlane G., Chuche J., Pommelet J.C., Cheikh A.B., Plisnier M. and Flammang R. (1991). 2,5-Dithiacyclopentylideneketene and Ethenedithione, S=C=C=S, Generated by Flash Vacuum Pyrolysis. Journal of the American Chemical Society, 113 (8), 3130-3135. doi: 10.1021/ja00008a048
Conference Papers
Kelly, Brian D., McLeod, Victoria, Walker, Rachael, Schreuders, Jeannette, Jackson, Susan, Giannis, Michael, Dietinger, Christine, Xia, Shirley, Cargill, Anne, Seta, Aynaz, Hufton, Richard, Heery, Graham, Cullinane, Carleen and Owen, David J. (2020). Anti-cancer activity of a SN-38 nanoparticle, DEP® irinotecan, in human colon cancer xenograft models. AACR Annual Meeting 2020, Philadelphia, PA United States, 27-28 April 2020. Philadelphia, PA United States: American Association for Cancer Research (AACR). doi: 10.1158/1538-7445.am2020-1715
Ashford, Marianne B., Balachander, Srividya B., Graham, Lorraine, Grant, Iain, Gibbons, Francis D., Hill, Kathryn J., Harmer, Alexander R., Gales, Sonya, Redmond, Sean, Kelly, Brian, McCoull, William, Wen, Shenghua, Wild, Martin, Gangl, Eric, Owen, David J. and Davies, Barry R. (2020). Design and optimization of a dendrimer-conjugated dual Bcl-2/Bcl-xL inhibitor, AZD0466, with improved therapeutic index. AACR Annual Meeting 2020, Philadelphia, PA United States, 27-28 April 2020. Philadelphia, PA United States: American Association for Cancer Research. doi: 10.1158/1538-7445.am2020-1718
Kelly, Brian D., McLeod, Victoria, Walker, Rachael, Schreuders, Jeannette, Jackson, Susan, Giannis, Michael, Dietinger, Christine, Reitano, Pauline, Pathak, Rashmi, Xia, Shirley, Cargill, Anne, Seta, Aynaz, Hufton, Richard, Heery, Graham, Cullinane, Carleen and Owen, David J. (2020). Anticancer activity of the taxane nanoparticles, DEP® docetaxel and DEP® cabazitaxel. AACR Annual Meeting 2020, Philadelphia, PA United States, 27-28 April 2020. Philadelphia, PA United States: American Association for Cancer Research. doi: 10.1158/1538-7445.am2020-1716
Patents
Owen, David (2017). Macromolecules.