Dr Shayli Moradi
Research Specialist - In Vitro Protein Translation
Protein Expression Facility
+61 7 3346 3498
Research Specialist - Analytics and Characterisation / In Vitro Protein Expression
Expertise
- Cell-free in vitro protein expression
- Protein analytics and characterisation
Book Chapters
Varasteh Moradi, Shayli, Johnston, Wayne A., Parker, Jake and Alexandrov, Kirill (2021). Leishmania tarentolae cell-free based approach for rapid antibody: antigen interaction analysis. Recombinant Protein Expression: Prokaryotic Hosts and Cell-Free Systems. (pp. 391-409) edited by Anna Marie Pyle and David W. Christianson. Cambridge, MA, USA: Academic Press. doi: 10.1016/bs.mie.2021.06.017
Johnston, Wayne A., Varasteh Moradi, Shayli and Alexandrov, Kirill (2019). Adaption of the Leishmania cell-free expression system to high-throughput analysis of protein interactions. High-throughput protein production and purification. (pp. 403-421) edited by Renaud Vincentelli. New York, NY, United States: Humana Press. doi: 10.1007/978-1-4939-9624-7_19
Journal Articles
Alfaro-Palma, Juan, Johnston, Wayne A., Behrendorff, James, Cui, Zhenling, Moradi, Shayli Varasteh and Alexandrov, Kirill (2024). Development of Lyophilized Eukaryotic Cell-Free Protein Expression System Based on Leishmania tarentolae. ACS Synthetic Biology, 13 (2), 449-456. doi: 10.1021/acssynbio.3c00428
Moradi, Shayli Varasteh, Wu, Yue, Walden, Patricia, Cui, Zhenling, Johnston, Wayne A., Petrov, Dmitri and Alexandrov, Kirill (2023). In vitro reconstitution and analysis of SARS-CoV-2/host protein-protein interactions. ACS Omega, 8 (28), 25009-25019. doi: 10.1021/acsomega.3c01625
Das Gupta, Kaustav, Ramnath, Divya, von Pein, Jessica B., Curson, James E. B., Wang, Yizhuo, Abrol, Rishika, Kakkanat, Asha, Moradi, Shayli Varasteh, Gunther, Kimberley S., Murthy, Ambika M. V., Stocks, Claudia J., Kapetanovic, Ronan, Reid, Robert C., Iyer, Abishek, Ilka, Zoe C., Nauseef, William M., Plan, Manuel, Luo, Lin, Stow, Jennifer L., Schroder, Kate, Karunakaran, Denuja, Alexandrov, Kirill, Shakespear, Melanie R., Schembri, Mark A., Fairlie, David P. and Sweet, Matthew J. (2023). HDAC7 is an immunometabolic switch triaging danger signals for engagement of antimicrobial versus inflammatory responses in macrophages. Proceedings of the National Academy of Sciences, 120 (4) e2212813120, 1-12. doi: 10.1073/pnas.2212813120
Guo, Zhong, Parakra, Rinky D., Xiong, Ying, Johnston, Wayne A., Walden, Patricia, Edwardraja, Selvakumar, Moradi, Shayli Varasteh, Ungerer, Jacobus P. J., Ai, Hui-wang, Phillips, Jonathan J. and Alexandrov, Kirill (2022). Engineering and exploiting synthetic allostery of NanoLuc luciferase. Nature Communications, 13 (1) 789, 789. doi: 10.1038/s41467-022-28425-2
Wu, Yue, Cui, Zhenling, Huang, Yen-Hua, de Veer, Simon J., Aralov, Andrey V., Guo, Zhong, Moradi, Shayli V., Hinton, Alexandra O., Deuis, Jennifer R., Guo, Shaodong, Chen, Kai-En, Collins, Brett M., Vetter, Irina, Herzig, Volker, Jones, Alun, Cooper, Matthew A., King, Glenn F., Craik, David J., Alexandrov, Kirill and Mureev, Sergey (2022). Towards a generic prototyping approach for therapeutically-relevant peptides and proteins in a cell-free translation system. Nature Communications, 13 (1) 260, 260. doi: 10.1038/s41467-021-27854-9
Lo, Harriet P., Lim, Ye-Wheen, Xiong, Zherui, Martel, Nick, Ferguson, Charles, Ariotti, Nicholas, Giacomotto, Jean, Rae, James, Floetenmeyer, Matthias, Moradi, Shayli Varasteh, Gao, Ya, Tillu, Vikas A., Xia, Di, Wang, Huang, Rahnama, Samira, Nixon, Susan J., Bastiani, Michele, Day, Ryan D., Smith, Kelly A., Palpant, Nathan J., Johnston, Wayne A., Alexandrov, Kirill, Collins, Brett M., Hall, Thomas E. and Parton, Robert G. (2021). Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle. Journal of Cell Biology, 220 (12) e201905065. doi: 10.1083/jcb.201905065
Souilmi, Yassine, Lauterbur, M. Elise, Tobler, Ray, Huber, Christian D., Johar, Angad S., Moradi, Shayli Varasteh, Johnston, Wayne A., Krogan, Nevan J., Alexandrov, Kirill and Enard, David (2021). An ancient viral epidemic involving host coronavirus interacting genes more than 20,000 years ago in East Asia. Current Biology, 31 (16), 3504-3514.e9. doi: 10.1016/j.cub.2021.05.067
Das Gupta, Kaustav, Shakespear, Melanie R., Curson, James E.B., Murthy, Ambika M.V., Iyer, Abishek, Hodson, Mark P., Ramnath, Divya, Tillu, Vikas A., von Pein, Jessica B., Reid, Robert C., Tunny, Kathryn, Hohenhaus, Daniel M., Moradi, Shayli Varasteh, Kelly, Gregory M., Kobayashi, Takumi, Gunter, Jennifer H., Stevenson, Alexander J., Xu, Weijun, Luo, Lin, Jones, Alun, Johnston, Wayne A., Blumenthal, Antje, Alexandrov, Kirill, Collins, Brett M., Stow, Jennifer L., Fairlie, David P. and Sweet, Matthew J. (2020). Class IIa histone deacetylases drive toll-like receptor-inducible glycolysis and macrophage inflammatory responses via pyruvate kinase M2. Cell Reports, 30 (8), 2712-2728.e8. doi: 10.1016/j.celrep.2020.02.007
Varasteh Moradi, Shayli, Gagoski, Dejan, Mureev, Sergey, Walden, Patricia, McMahon, Kerrie-Ann, Parton, Robert G., Johnston, Wayne A. and Alexandrov, Kirill (2020). Mapping interactions among cell-free expressed Zirka virus proteins. Journal of Proteome Research, 19 (4) acs.jproteome.9b00771, 1522-1532. doi: 10.1021/acs.jproteome.9b00771
McMahon, Kerrie-Ann, Wu, Yeping, Gambin, Yann, Sierecki, Emma, Tillu, Vikas A., Hall, Thomas, Martel, Nick, Okano, Satomi, Moradi, Shayli Varasteh, Ruelcke, Jayde E., Ferguson, Charles, Yap, Alpha S., Alexandrov, Kirill, Hill, Michelle M. and Parton, Robert G. (2019). Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis. Nature Communications, 10 (1) 3279, 3279. doi: 10.1038/s41467-019-11111-1
Moradi, Shayli Varasteh, Hussein, Waleed M., Varamini, Pegah, Simerska, Pavla and Toth, Istvan (2016). Glycosylation, an effective synthetic strategy to improve the bioavailability of therapeutic peptides. Chemical Science, 7 (4), 2492-2500. doi: 10.1039/C5SC04392A
Varasteh Moradi, Shayli, Varamini, Pegah, Steyn, Frederik and Toth, Istvan (2015). In vivo pharmacological evaluation of a lactose-conjugated luteinizing hormone releasing hormone analogue. International Journal of Pharmaceutics, 495 (1), 106-111. doi: 10.1016/j.ijpharm.2015.08.095
Moradi, Shayli Varasteh, Varamini, Pegah and Toth, Istvan (2015). Evaluation of the biological properties and the enzymatic stability of glycosylated luteinizing hormone-releasing hormone analogs. AAPS Journal, 17 (5), 1135-1143. doi: 10.1208/s12248-015-9769-x
Moradi, Shayli Varasteh, Varamini, Pegah and Toth, Istvan (2014). The transport and efflux of glycosylated luteinising hormone-releasing hormone analogues in Caco-2 cell model: contributions of glucose transporters and efflux systems. Journal of Pharmaceutical Sciences, Early View (10), 3217-3224. doi: 10.1002/jps.24120
Moradi, Shayli Varasteh, Mansfeld, Friederike M. and Toth, Istvan (2013). Synthesis and in vitro evaluation of glycosyl derivatives of luteinizing hormone-releasing hormone (LHRH). Bioorganic and Medicinal Chemistry, 21 (14), 4259-4265. doi: 10.1016/j.bmc.2013.04.068
Conference Papers
Moradi, S. V., Varamini, P. and Toth, I. (2014). Glycosylation of luteninzing hormone releasing hormoe: towards the development of orally active peptide. Dr. Bert L. Schram Young Investigators' Mini-Symposium. 33EPS 2014: 33rd European Peptide Symposium, Sofia, Bulgaria, 31 August-5 September, 2014. Chichester, West Sussex, United Kingdom: John Wiley & Sons. doi: 10.1002/psc.2686
Preprint
Fonseka, Sachini, Weger, Benjamin D., Weger, Meltem, Martel, Nick, Hall, Thomas Edward, Varasteh Moradi, Shayli, Gabriel, Christian H., Kramer, Achim, Ferguson, Charles, Fernández-Rojo, Manuel A., Alexandrov, Kirill, Rawashdeh, Oliver, McMahon, Kerrie-Ann, Gachon, Frederic and Parton, Robert G. (2022). A role for caveolar proteins in regulation of the circadian clock.
Thesis
Varasteh Moradi, Shayli (2015). Improving the bioavailability and stability of luteinizing hormone-releasing hormone (LHRH) analogues. PhD Thesis, School of Chemistry and Molecular Biosciences, The University of Queensland. doi: 10.14264/uql.2015.917