Chart demonstrating advantages and drawbacks of in vivo expression systems

Each in vivo system has benefits and drawbacks with respect to their capacity for recombinant protein production.

The figure on the right illustrates some of the key advantages and disadvantages of the four major systems used in PEF.

Expression Optimisation

PEF offers a small-scale expression screen in each system that is designed to test different expression parameters to find an optimal condition. Users can request specific conditions or allow PEF to suggest a set of conditions to use in each screen. For small-scale expression services, please contact us for a quote.

Large-scale Expression

PEF offers large-scale expression in bacteria, yeast, insect and mammalian cell systems. Culture volumes from 400 mL to 10 L are available in shake flask and from 2 L to 20 L in a stirred-tank bioreactor or up to 25 L in a Wave bioreactor. For large-scale expression services, please contact us for a quote.