Molecular Engineering Library

PEF has extensive knowledge in molecular engineering techniques, which include molecular cloning, site-directed mutagenesis (SDM), vector engineering and plasmid isolation. Find out how each technique may accelerate your research.


Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression.
Unger, T., Jacobovitch, Y., Dantes, A., Bernheim, R. & Peleg, Y. Journal of structural biology 172, 34-44 (2010).

Circular polymerase extension cloning of complex gene libraries and pathways.
Quan, J. & Tian, J. PloS one 4, e6441 (2009).

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications
Berrow, N.S. et al. Nucleic acids research 35, e45 (2007).

Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.
Li, M.Z. & Elledge, S.J. Nat Meth 4, 251-256 (2007).

A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production.
Cabrita, L.D., Dai, W. & Bottomley, S.P. BMC biotechnology 6, 12 (2006).